The 2 halves of the band underwent a video call with entertainment reporter Brooke Boney from their homes in Los Angeles, where they live, and shocked fans with their changing accents.
Talking about their new album Calm topping the charts, you can hear a definite change in the way they speak to how they usually sound, confusing fans further.
"It makes me a little sad that we are losing the Australian accents," one fan said.
Another added: "It's so weird hearing 5SOS speak nowadays because they sound so intensely American and have lost their Aussie accents."
"Okay so 5SOS is one of my favourite bands but I don't watch videos of them talking in interviews anymore (I was obsessed and used to back in 2015) and I can't believe they have absolutely no Aussie accents anymore it's crazy to me I can still hear their old voices in my head."
In 2018, the boys were once again slammed on Twitter for their strange accents after appearing on The Project, with some fans accusing the of 'faking' their accents.
"Can 5SOS stop with the fake American accents ya from Western Sydney..." one viewer tweeted.
"5SOS sound SO American! RIP their Aussie accents," another tweeted, with a laughing emoji.
The boys have explained why their accents are so strange.
"I think because we moved away at 16/17 (years of age) and we moved to England so...that affected our accents when we were younger because we learnt to sing from a Danish woman," Ashton revealed.
"Who taught us to say things like lemon instead of 'lemon' (he said in an accent)... we used to speak like that," Ashton added.
"Our accents have evolved over time...back and forth and it's just a big mess ...singing voices change, your accent changes because we've lived away for nearly 7 years," he finished.
Michael Clifford also told The Kyle and Jackie O Show that same year that: "I think all of us kind of have to chameleon a little bit into other accents.
"People in other countries just don't understand Australians and that's a fact."
He added: "If anyone from Australia has travelled overseas, no one knows what the f**k we're talking about, so you have to change your accent and you have to adapt."